ClickTech EdU Cell Proliferation Kit 647 (100 Assays) for IM IV [BCK-EdU647IM100+IV]_Baseclick - 코아사이언스
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ClickTech EdU Cell Proliferation Kit 647 (100 Assays) for IM IV
In vivo EdU Cell Proliferation Kit for Imaging
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Size
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Cat.#
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1 Kit size S (+50 mg EdU)
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BCK-EdU647IM100+IV-S
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1 Kit size M (+500 mg EdU)
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BCK-EdU647IM100+IV-M
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1 Kit size L (+1000 mg EdU)
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BCK-EdU647IM100+IV-L
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Information
EdU in vivo cell proliferation Kit provides a superior alternative to BrdU and [3H]-thymidine assays for detection of cell proliferation in animals.
In principal, EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog, is administered to animals. EdU is incorporated into DNA during DNA synthesis and has a clickable functionality.
Detection of cell division in tissues can be performed using microscopy, flow cytometry or in high throughput screening methods. The in vivo kits are thus designed in a sense that we provide additional EdU for the administering step. The three variants of baseclick EdU in vivo kits are:
1. Imaging kit (BCK-EdU-IM100+IV)
2. Flow cytometry kit (BCK-EdU-FC50+IV)
3. High throughput screening – HTS kit (BCK-EdU-HTS2+IV)
LITERATURE
An efficient protocol for in vivo labeling of proliferating epithelial cells, C. Michel et al., 2018, J. Immunol. Methods, Vol. 457, p. 82-86.
https://doi.org/10.1016/j.jim.2018.03.015
Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterility, L. Wang et al., 2018, eLife, Vol. 7, p. e42511.
https://doi.org/10.7554/eLife.42511
FXR Regulates Intestinal Cancer Stem Cell Proliferation, T. Fu et al., 2019, Cell, Vol. 176, p. 1098-1112.
https://doi.org/10.1016/j.cell.2019.01.036
Chemical Properties
- Shelf Life: 12 months unopened after receipt
- Storage Conditions: 2-8°C
- Physical State: kit system made of different components
- CAS Number: n.a.
- Excitation (max): 643 nm
- Emission (max): 662 nm
- Ɛ (max): 250.000 cm-1M-1
- Preparation/Handling: please see user manual of the kit
Documentation
For research use only!
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