"Prote-in" Transfection Reagent - Peptide-base Protein Transfection Reagent for Cells [160-0526-0, 160-0518-0]_Funakoshi - 코아사이언스
Peptide-base Protein Transfection Reagent for Cells
"Prote-in" Transfection Reagent
Ready to use and suitable replacement of trypsin
Cat.# 160-0526-0 / Size: 6 tests
Cat.# 160-0518-0 / Size: 24 tests
본 상품은 펩타이드를 기반으로 한 세포 내로의 단백질 형질전환 시약입니다. 세포막 투과정이 매우 우수하며 단백질-운반체 복합체 형성이 잘 됩니다.
"Prote-in" is a peptide-base protein transfection reagent for cells. This reagent has the excellent ability of membrane penetration and formulation of protein-transporter complex.
Peptide transporter "Prote-in" consists of 3 region of peptide sequences - Hydrophobic region, Linker and Cell Penetrating Peptide (CPP) area. Hydrophobic region binds to Proteins. CPP region helps to corporate the protein-transporter complex into cytoplasm by binding to heparan sulfate proteoglycan on the cell surface. As transporter and protein do not bind covalently, protein function is maintained.
Features of "Prote-in"
- Low cytotoxicity
- Rapid - Complex is structured in an hour, and transfection is completed in 3 hours.
- Versatility - Can be used for both Adherent and Non-adherent Cells.
Principle of "Prote-in"
Protocol Outline
Example of Use
HeLa-S3 Cell Line (Human Uterus Cancer : Adherent Cell)
FITC labeled Antibody : Anti-Sheep IgG (Whole Molecule), FITC Conjugate, Donkey Polyclonal (SIGMA)
Examples of Cells Transfected Successfully
| Adherent Cells | Non - Adherent Cells |
|---|---|
| HeLa-S3 cell line | K562 cell line |
| WiDr cell line | Jurkat cell line |
| HepG2 cell line | HL60 cell line |
| MCF-7 cell line | P388 cell |
| Balb3T3 cell line | P3U1 cell |
| B16 cell line | |
| SVT2 cell line | |
| A10 cell line |
Comparison Data
References
1. Eisaku et al., High efficient delivery of p16 antitumor peptide into aggressive leukemia/lymphoma cells using a novel transporter system. Mol. Cancer Ther., 3(12),1623-1630 (2004).
2. Eisaku et al., Potent synergy of dual antitumor peptides for growth suppression of human glioblastoma cell lines. Mol. Cancer Ther., 7(6), 1461-1471 (2008).
* FOR RESEARCH USE onLY. NOT FOR DIAGNOSTIC USE.
